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Three prolyl endopeptidase (PIP) inhibitors were isolated from the methanolic extract of green tea leaves. They were identified as (-)-epigallocatechin gallate, (-)-epicatechin gallate, and (+)-gallocatechin gallate with the IC50 values of 1.42×10-4 mM, 1.02×10-2 mM, and 1.09×10-4 mM, respectively. They were non-competitive with a substrate in Dixon plots and did not show any significant effects against other serine proteases such as elastase, trypsin, and chymotrypsin, suggesting that they were relatively specific inhibitors against PIP The isolated compounds are expected to be useful for preventing and curing of Alzheimer’s disease.
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